Fast and accurate data collection for macromolecular crystallography using the JUNGFRAU detector.
Identifieur interne : 000068 ( Main/Exploration ); précédent : 000067; suivant : 000069Fast and accurate data collection for macromolecular crystallography using the JUNGFRAU detector.
Auteurs : Filip Leonarski [Suisse] ; Sophie Redford [Suisse] ; Aldo Mozzanica [Suisse] ; Carlos Lopez-Cuenca [Suisse] ; Ezequiel Panepucci [Suisse] ; Karol Nass [Suisse] ; Dmitry Ozerov [Suisse] ; Laura Vera [Suisse] ; Vincent Olieric [Suisse] ; Dominik Buntschu [Suisse] ; Roman Schneider [Suisse] ; Gemma Tinti [Suisse] ; Erik Froejdh [Suisse] ; Kay Diederichs [Allemagne] ; Oliver Bunk [Suisse] ; Bernd Schmitt [Suisse] ; Meitian Wang [Suisse]Source :
- Nature methods [ 1548-7105 ] ; 2018.
Descripteurs français
- KwdFr :
- Antigènes CD13 (composition chimique), Collecte de données (méthodes), Conception d'appareillage (MeSH), Cristallographie aux rayons X (instrumentation), Cristallographie aux rayons X (méthodes), Humains (MeSH), Lysozyme (composition chimique), Modèles moléculaires (MeSH), Structures macromoléculaires (composition chimique), Synchrotrons (instrumentation).
- MESH :
- composition chimique : Antigènes CD13, Lysozyme, Structures macromoléculaires.
- méthodes : Collecte de données, Cristallographie aux rayons X.
- instrumentation : Conception d'appareillage, Cristallographie aux rayons X, Humains, Modèles moléculaires, Synchrotrons.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : CD13 Antigens, Macromolecular Substances, Muramidase.
- instrumentation : Crystallography, X-Ray, Synchrotrons.
- methods : Crystallography, X-Ray, Data Collection.
- Equipment Design, Humans, Models, Molecular.
Abstract
The accuracy of X-ray diffraction data is directly related to how the X-ray detector records photons. Here we describe the application of a direct-detection charge-integrating pixel-array detector (JUNGFRAU) in macromolecular crystallography (MX). JUNGFRAU features a uniform response on the subpixel level, linear behavior toward high photon rates, and low-noise performance across the whole dynamic range. We demonstrate that these features allow accurate MX data to be recorded at unprecedented speed. We also demonstrate improvements over previous-generation detectors in terms of data quality, using native single-wavelength anomalous diffraction (SAD) phasing, for thaumatin, lysozyme, and aminopeptidase N. Our results suggest that the JUNGFRAU detector will substantially improve the performance of synchrotron MX beamlines and equip them for future synchrotron light sources.
DOI: 10.1038/s41592-018-0143-7
PubMed: 30275593
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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R" first="Roman" last="Schneider">Roman Schneider</name><affiliation wicri:level="1"><nlm:affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Swiss Light Source, Paul Scherrer Institute, Villigen</wicri:regionArea><wicri:noRegion>Villigen</wicri:noRegion></affiliation></author><author><name sortKey="Tinti, Gemma" sort="Tinti, Gemma" uniqKey="Tinti G" first="Gemma" last="Tinti">Gemma Tinti</name><affiliation wicri:level="1"><nlm:affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Swiss Light Source, Paul Scherrer Institute, Villigen</wicri:regionArea><wicri:noRegion>Villigen</wicri:noRegion></affiliation></author><author><name sortKey="Froejdh, Erik" sort="Froejdh, Erik" uniqKey="Froejdh E" first="Erik" last="Froejdh">Erik Froejdh</name><affiliation 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uniqKey="Bunk O" first="Oliver" last="Bunk">Oliver Bunk</name><affiliation wicri:level="1"><nlm:affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Swiss Light Source, Paul Scherrer Institute, Villigen</wicri:regionArea><wicri:noRegion>Villigen</wicri:noRegion></affiliation></author><author><name sortKey="Schmitt, Bernd" sort="Schmitt, Bernd" uniqKey="Schmitt B" first="Bernd" last="Schmitt">Bernd Schmitt</name><affiliation wicri:level="1"><nlm:affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland. bernd.schmitt@psi.ch.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Swiss Light Source, Paul Scherrer Institute, Villigen</wicri:regionArea><wicri:noRegion>Villigen</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Meitian" sort="Wang, Meitian" uniqKey="Wang M" first="Meitian" last="Wang">Meitian Wang</name><affiliation wicri:level="1"><nlm:affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland. meitian.wang@psi.ch.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Swiss Light Source, Paul Scherrer Institute, Villigen</wicri:regionArea><wicri:noRegion>Villigen</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:30275593</idno><idno type="pmid">30275593</idno><idno type="doi">10.1038/s41592-018-0143-7</idno><idno type="wicri:Area/Main/Corpus">000054</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000054</idno><idno type="wicri:Area/Main/Curation">000054</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000054</idno><idno type="wicri:Area/Main/Exploration">000054</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Fast and accurate data collection for macromolecular crystallography using the JUNGFRAU detector.</title><author><name sortKey="Leonarski, Filip" sort="Leonarski, Filip" uniqKey="Leonarski F" first="Filip" last="Leonarski">Filip Leonarski</name><affiliation wicri:level="1"><nlm:affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Swiss Light Source, Paul Scherrer Institute, Villigen</wicri:regionArea><wicri:noRegion>Villigen</wicri:noRegion></affiliation></author><author><name sortKey="Redford, Sophie" sort="Redford, Sophie" uniqKey="Redford S" first="Sophie" last="Redford">Sophie Redford</name><affiliation wicri:level="1"><nlm:affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Swiss Light Source, Paul Scherrer Institute, Villigen</wicri:regionArea><wicri:noRegion>Villigen</wicri:noRegion></affiliation></author><author><name sortKey="Mozzanica, Aldo" sort="Mozzanica, Aldo" uniqKey="Mozzanica A" first="Aldo" last="Mozzanica">Aldo Mozzanica</name><affiliation wicri:level="1"><nlm:affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Swiss Light Source, Paul Scherrer Institute, Villigen</wicri:regionArea><wicri:noRegion>Villigen</wicri:noRegion></affiliation></author><author><name sortKey="Lopez Cuenca, Carlos" sort="Lopez Cuenca, Carlos" uniqKey="Lopez Cuenca C" first="Carlos" last="Lopez-Cuenca">Carlos Lopez-Cuenca</name><affiliation wicri:level="1"><nlm:affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Swiss Light Source, Paul Scherrer Institute, Villigen</wicri:regionArea><wicri:noRegion>Villigen</wicri:noRegion></affiliation></author><author><name sortKey="Panepucci, Ezequiel" sort="Panepucci, Ezequiel" uniqKey="Panepucci E" first="Ezequiel" last="Panepucci">Ezequiel Panepucci</name><affiliation wicri:level="1"><nlm:affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Swiss Light Source, Paul Scherrer Institute, Villigen</wicri:regionArea><wicri:noRegion>Villigen</wicri:noRegion></affiliation></author><author><name sortKey="Nass, Karol" sort="Nass, Karol" uniqKey="Nass K" first="Karol" last="Nass">Karol Nass</name><affiliation wicri:level="1"><nlm:affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Swiss Light Source, Paul Scherrer Institute, Villigen</wicri:regionArea><wicri:noRegion>Villigen</wicri:noRegion></affiliation></author><author><name sortKey="Ozerov, Dmitry" sort="Ozerov, Dmitry" uniqKey="Ozerov D" first="Dmitry" last="Ozerov">Dmitry Ozerov</name><affiliation wicri:level="1"><nlm:affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Swiss Light Source, Paul Scherrer Institute, Villigen</wicri:regionArea><wicri:noRegion>Villigen</wicri:noRegion></affiliation></author><author><name sortKey="Vera, Laura" sort="Vera, Laura" uniqKey="Vera L" first="Laura" last="Vera">Laura Vera</name><affiliation wicri:level="1"><nlm:affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Swiss Light Source, Paul Scherrer Institute, Villigen</wicri:regionArea><wicri:noRegion>Villigen</wicri:noRegion></affiliation></author><author><name sortKey="Olieric, Vincent" sort="Olieric, Vincent" uniqKey="Olieric V" first="Vincent" last="Olieric">Vincent Olieric</name><affiliation wicri:level="1"><nlm:affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Swiss Light Source, Paul Scherrer Institute, Villigen</wicri:regionArea><wicri:noRegion>Villigen</wicri:noRegion></affiliation></author><author><name sortKey="Buntschu, Dominik" sort="Buntschu, Dominik" uniqKey="Buntschu D" first="Dominik" last="Buntschu">Dominik Buntschu</name><affiliation wicri:level="1"><nlm:affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Swiss Light Source, Paul Scherrer Institute, Villigen</wicri:regionArea><wicri:noRegion>Villigen</wicri:noRegion></affiliation></author><author><name sortKey="Schneider, Roman" sort="Schneider, Roman" uniqKey="Schneider R" first="Roman" last="Schneider">Roman Schneider</name><affiliation wicri:level="1"><nlm:affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Swiss Light Source, Paul Scherrer Institute, Villigen</wicri:regionArea><wicri:noRegion>Villigen</wicri:noRegion></affiliation></author><author><name sortKey="Tinti, Gemma" sort="Tinti, Gemma" uniqKey="Tinti G" first="Gemma" last="Tinti">Gemma Tinti</name><affiliation wicri:level="1"><nlm:affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Swiss Light Source, Paul Scherrer Institute, Villigen</wicri:regionArea><wicri:noRegion>Villigen</wicri:noRegion></affiliation></author><author><name sortKey="Froejdh, Erik" sort="Froejdh, Erik" uniqKey="Froejdh E" first="Erik" last="Froejdh">Erik Froejdh</name><affiliation wicri:level="1"><nlm:affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Swiss Light Source, Paul Scherrer Institute, Villigen</wicri:regionArea><wicri:noRegion>Villigen</wicri:noRegion></affiliation></author><author><name sortKey="Diederichs, Kay" sort="Diederichs, Kay" uniqKey="Diederichs K" first="Kay" last="Diederichs">Kay Diederichs</name><affiliation wicri:level="3"><nlm:affiliation>Fachbereich Biologie, Universität Konstanz, Konstanz, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Fachbereich Biologie, Universität Konstanz, Konstanz</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Fribourg-en-Brisgau</region><settlement type="city">Constance (Allemagne)</settlement></placeName></affiliation></author><author><name sortKey="Bunk, Oliver" sort="Bunk, Oliver" uniqKey="Bunk O" first="Oliver" last="Bunk">Oliver Bunk</name><affiliation wicri:level="1"><nlm:affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Swiss Light Source, Paul Scherrer Institute, Villigen</wicri:regionArea><wicri:noRegion>Villigen</wicri:noRegion></affiliation></author><author><name sortKey="Schmitt, Bernd" sort="Schmitt, Bernd" uniqKey="Schmitt B" first="Bernd" last="Schmitt">Bernd Schmitt</name><affiliation wicri:level="1"><nlm:affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland. bernd.schmitt@psi.ch.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Swiss Light Source, Paul Scherrer Institute, Villigen</wicri:regionArea><wicri:noRegion>Villigen</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Meitian" sort="Wang, Meitian" uniqKey="Wang M" first="Meitian" last="Wang">Meitian Wang</name><affiliation wicri:level="1"><nlm:affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland. meitian.wang@psi.ch.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Swiss Light Source, Paul Scherrer Institute, Villigen</wicri:regionArea><wicri:noRegion>Villigen</wicri:noRegion></affiliation></author></analytic><series><title level="j">Nature methods</title><idno type="eISSN">1548-7105</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>CD13 Antigens (chemistry)</term><term>Crystallography, X-Ray (instrumentation)</term><term>Crystallography, X-Ray (methods)</term><term>Data Collection (methods)</term><term>Equipment Design (MeSH)</term><term>Humans (MeSH)</term><term>Macromolecular Substances (chemistry)</term><term>Models, Molecular (MeSH)</term><term>Muramidase (chemistry)</term><term>Synchrotrons (instrumentation)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antigènes CD13 (composition chimique)</term><term>Collecte de données (méthodes)</term><term>Conception d'appareillage (MeSH)</term><term>Cristallographie aux rayons X (instrumentation)</term><term>Cristallographie aux rayons X (méthodes)</term><term>Humains (MeSH)</term><term>Lysozyme (composition chimique)</term><term>Modèles moléculaires (MeSH)</term><term>Structures macromoléculaires (composition chimique)</term><term>Synchrotrons (instrumentation)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>CD13 Antigens</term><term>Macromolecular Substances</term><term>Muramidase</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Antigènes CD13</term><term>Lysozyme</term><term>Structures macromoléculaires</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Crystallography, X-Ray</term><term>Synchrotrons</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Crystallography, X-Ray</term><term>Data Collection</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Collecte de données</term><term>Cristallographie aux rayons X</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Equipment Design</term><term>Humans</term><term>Models, Molecular</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="fr"><term>Conception d'appareillage</term><term>Cristallographie aux rayons X</term><term>Humains</term><term>Modèles moléculaires</term><term>Synchrotrons</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The accuracy of X-ray diffraction data is directly related to how the X-ray detector records photons. Here we describe the application of a direct-detection charge-integrating pixel-array detector (JUNGFRAU) in macromolecular crystallography (MX). JUNGFRAU features a uniform response on the subpixel level, linear behavior toward high photon rates, and low-noise performance across the whole dynamic range. We demonstrate that these features allow accurate MX data to be recorded at unprecedented speed. We also demonstrate improvements over previous-generation detectors in terms of data quality, using native single-wavelength anomalous diffraction (SAD) phasing, for thaumatin, lysozyme, and aminopeptidase N. Our results suggest that the JUNGFRAU detector will substantially improve the performance of synchrotron MX beamlines and equip them for future synchrotron light sources.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30275593</PMID><DateCompleted><Year>2019</Year><Month>05</Month><Day>13</Day></DateCompleted><DateRevised><Year>2019</Year><Month>08</Month><Day>29</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1548-7105</ISSN><JournalIssue CitedMedium="Internet"><Volume>15</Volume><Issue>10</Issue><PubDate><Year>2018</Year><Month>10</Month></PubDate></JournalIssue><Title>Nature methods</Title><ISOAbbreviation>Nat Methods</ISOAbbreviation></Journal><ArticleTitle>Fast and accurate data collection for macromolecular crystallography using the JUNGFRAU detector.</ArticleTitle><Pagination><MedlinePgn>799-804</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/s41592-018-0143-7</ELocationID><Abstract><AbstractText>The accuracy of X-ray diffraction data is directly related to how the X-ray detector records photons. Here we describe the application of a direct-detection charge-integrating pixel-array detector (JUNGFRAU) in macromolecular crystallography (MX). JUNGFRAU features a uniform response on the subpixel level, linear behavior toward high photon rates, and low-noise performance across the whole dynamic range. We demonstrate that these features allow accurate MX data to be recorded at unprecedented speed. We also demonstrate improvements over previous-generation detectors in terms of data quality, using native single-wavelength anomalous diffraction (SAD) phasing, for thaumatin, lysozyme, and aminopeptidase N. Our results suggest that the JUNGFRAU detector will substantially improve the performance of synchrotron MX beamlines and equip them for future synchrotron light sources.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Leonarski</LastName><ForeName>Filip</ForeName><Initials>F</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-2563-7169</Identifier><AffiliationInfo><Affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Redford</LastName><ForeName>Sophie</ForeName><Initials>S</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-1828-9116</Identifier><AffiliationInfo><Affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mozzanica</LastName><ForeName>Aldo</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lopez-Cuenca</LastName><ForeName>Carlos</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Panepucci</LastName><ForeName>Ezequiel</ForeName><Initials>E</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-3388-997X</Identifier><AffiliationInfo><Affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nass</LastName><ForeName>Karol</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ozerov</LastName><ForeName>Dmitry</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vera</LastName><ForeName>Laura</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Olieric</LastName><ForeName>Vincent</ForeName><Initials>V</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-0533-7222</Identifier><AffiliationInfo><Affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Buntschu</LastName><ForeName>Dominik</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schneider</LastName><ForeName>Roman</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tinti</LastName><ForeName>Gemma</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Froejdh</LastName><ForeName>Erik</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Diederichs</LastName><ForeName>Kay</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Fachbereich Biologie, Universität Konstanz, Konstanz, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bunk</LastName><ForeName>Oliver</ForeName><Initials>O</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-6563-4053</Identifier><AffiliationInfo><Affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schmitt</LastName><ForeName>Bernd</ForeName><Initials>B</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-5778-0680</Identifier><AffiliationInfo><Affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland. bernd.schmitt@psi.ch.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Meitian</ForeName><Initials>M</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-5340-3036</Identifier><AffiliationInfo><Affiliation>Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland. meitian.wang@psi.ch.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>10</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Nat Methods</MedlineTA><NlmUniqueID>101215604</NlmUniqueID><ISSNLinking>1548-7091</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D046911">Macromolecular Substances</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.17</RegistryNumber><NameOfSubstance UI="D009113">Muramidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.11.2</RegistryNumber><NameOfSubstance UI="D018826">CD13 Antigens</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="CommentIn"><RefSource>Nat Methods. 2018 Oct;15(10):774-775</RefSource><PMID Version="1">30275571</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D018826" MajorTopicYN="N">CD13 Antigens</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018360" MajorTopicYN="N">Crystallography, X-Ray</DescriptorName><QualifierName UI="Q000295" MajorTopicYN="Y">instrumentation</QualifierName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003625" MajorTopicYN="N">Data Collection</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004867" MajorTopicYN="N">Equipment Design</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D046911" MajorTopicYN="N">Macromolecular Substances</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008958" MajorTopicYN="N">Models, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009113" MajorTopicYN="N">Muramidase</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017356" MajorTopicYN="N">Synchrotrons</DescriptorName><QualifierName UI="Q000295" MajorTopicYN="Y">instrumentation</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>03</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>07</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>10</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>10</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>5</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30275593</ArticleId><ArticleId IdType="doi">10.1038/s41592-018-0143-7</ArticleId><ArticleId IdType="pii">10.1038/s41592-018-0143-7</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li><li>Suisse</li></country><region><li>Bade-Wurtemberg</li><li>District de Fribourg-en-Brisgau</li></region><settlement><li>Constance (Allemagne)</li></settlement></list><tree><country name="Suisse"><noRegion><name sortKey="Leonarski, Filip" sort="Leonarski, Filip" uniqKey="Leonarski F" first="Filip" last="Leonarski">Filip Leonarski</name></noRegion><name sortKey="Bunk, Oliver" sort="Bunk, Oliver" uniqKey="Bunk O" first="Oliver" last="Bunk">Oliver Bunk</name><name sortKey="Buntschu, Dominik" sort="Buntschu, Dominik" uniqKey="Buntschu D" first="Dominik" last="Buntschu">Dominik Buntschu</name><name sortKey="Froejdh, Erik" sort="Froejdh, Erik" uniqKey="Froejdh E" first="Erik" last="Froejdh">Erik Froejdh</name><name sortKey="Lopez Cuenca, Carlos" sort="Lopez Cuenca, Carlos" uniqKey="Lopez Cuenca C" first="Carlos" last="Lopez-Cuenca">Carlos Lopez-Cuenca</name><name sortKey="Mozzanica, Aldo" sort="Mozzanica, Aldo" uniqKey="Mozzanica A" first="Aldo" last="Mozzanica">Aldo Mozzanica</name><name sortKey="Nass, Karol" sort="Nass, Karol" uniqKey="Nass K" first="Karol" last="Nass">Karol Nass</name><name sortKey="Olieric, Vincent" sort="Olieric, Vincent" uniqKey="Olieric V" first="Vincent" last="Olieric">Vincent Olieric</name><name sortKey="Ozerov, Dmitry" sort="Ozerov, Dmitry" uniqKey="Ozerov D" first="Dmitry" last="Ozerov">Dmitry Ozerov</name><name sortKey="Panepucci, Ezequiel" sort="Panepucci, Ezequiel" uniqKey="Panepucci E" first="Ezequiel" last="Panepucci">Ezequiel Panepucci</name><name sortKey="Redford, Sophie" sort="Redford, Sophie" uniqKey="Redford S" first="Sophie" last="Redford">Sophie Redford</name><name sortKey="Schmitt, Bernd" sort="Schmitt, Bernd" uniqKey="Schmitt B" first="Bernd" last="Schmitt">Bernd Schmitt</name><name sortKey="Schneider, Roman" sort="Schneider, Roman" uniqKey="Schneider R" first="Roman" last="Schneider">Roman Schneider</name><name sortKey="Tinti, Gemma" sort="Tinti, Gemma" uniqKey="Tinti G" first="Gemma" last="Tinti">Gemma Tinti</name><name sortKey="Vera, Laura" sort="Vera, Laura" uniqKey="Vera L" first="Laura" last="Vera">Laura Vera</name><name sortKey="Wang, Meitian" sort="Wang, Meitian" uniqKey="Wang M" first="Meitian" last="Wang">Meitian Wang</name></country><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Diederichs, Kay" sort="Diederichs, Kay" uniqKey="Diederichs K" first="Kay" last="Diederichs">Kay Diederichs</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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